Captured Proteins by Immobilized Ricinus Communis Agglutinin in Rabbit Plasma
نویسندگان
چکیده
The Sepharose CL-6B column was prepared to separate ricinus communis agglutinin (RCA) from the crude extract of castor beans due to the gel carrier contains a large amount of β-galactoside bond. RCA were immobilized on activated Sepharose 4B to make an immobilized RCA column, which was used to capture the proteins in rabbit plasma by affinity chromatography. Results showed that two polypeptides with molecular mass about 120 and 97 KDa were captured by RCA in rabbit plasma. The captured polypeptides were demonstrated to contain the carbohydrate. The method performed in this study is much useful in the separation of sugary proteins.
منابع مشابه
Ricin Trafficking in Plant and Mammalian Cells
Ricin is a heterodimeric plant protein that is potently toxic to mammalian and many other eukaryotic cells. It is synthesized and stored in the endosperm cells of maturing Ricinus communis seeds (castor beans). The ricin family has two major members, both, lectins, collectively known as Ricinus communis agglutinin ll (ricin) and Ricinus communis agglutinin l (RCA). These proteins are stored in ...
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